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type erbb2 expression construct  (Addgene inc)


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    Addgene inc type erbb2 expression construct
    Validation of successful ectopic overexpression of <t>ERBB2</t> in the CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines. ( A ) Relative ERBB2 expression in the normal colon cell lines CCD33 and CCD841 and the CRC cell lines HT29 and HCT116 as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the CCD33 cells. Data represent the mean ± SD of three independent experiments. ( B ) Relative ERBB2 expression in non-transfected and either empty pcDNA3 vector or pcDNA3- ERBB2 transfected HCT116, HT29, CCD33, and CCD841 cells as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the non-transfected cells. Data represent the mean ± SD of three independent experiments. *** p < 0.001; ns: not significant. ( C ) Same as B, but relative HER2 protein expression was determined in the different experimental conditions. Blots were re-probed with anti-β-Actin antibody to confirm equal loading across the lanes. The representative blots from three independent experiments are shown.
    Type Erbb2 Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type erbb2 expression construct/product/Addgene inc
    Average 93 stars, based on 59 article reviews
    type erbb2 expression construct - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Transcriptomic Changes Associated with ERBB2 Overexpression in Colorectal Cancer Implicate a Potential Role of the Wnt Signaling Pathway in Tumorigenesis"

    Article Title: Transcriptomic Changes Associated with ERBB2 Overexpression in Colorectal Cancer Implicate a Potential Role of the Wnt Signaling Pathway in Tumorigenesis

    Journal: Cancers

    doi: 10.3390/cancers15010130

    Validation of successful ectopic overexpression of ERBB2 in the CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines. ( A ) Relative ERBB2 expression in the normal colon cell lines CCD33 and CCD841 and the CRC cell lines HT29 and HCT116 as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the CCD33 cells. Data represent the mean ± SD of three independent experiments. ( B ) Relative ERBB2 expression in non-transfected and either empty pcDNA3 vector or pcDNA3- ERBB2 transfected HCT116, HT29, CCD33, and CCD841 cells as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the non-transfected cells. Data represent the mean ± SD of three independent experiments. *** p < 0.001; ns: not significant. ( C ) Same as B, but relative HER2 protein expression was determined in the different experimental conditions. Blots were re-probed with anti-β-Actin antibody to confirm equal loading across the lanes. The representative blots from three independent experiments are shown.
    Figure Legend Snippet: Validation of successful ectopic overexpression of ERBB2 in the CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines. ( A ) Relative ERBB2 expression in the normal colon cell lines CCD33 and CCD841 and the CRC cell lines HT29 and HCT116 as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the CCD33 cells. Data represent the mean ± SD of three independent experiments. ( B ) Relative ERBB2 expression in non-transfected and either empty pcDNA3 vector or pcDNA3- ERBB2 transfected HCT116, HT29, CCD33, and CCD841 cells as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the non-transfected cells. Data represent the mean ± SD of three independent experiments. *** p < 0.001; ns: not significant. ( C ) Same as B, but relative HER2 protein expression was determined in the different experimental conditions. Blots were re-probed with anti-β-Actin antibody to confirm equal loading across the lanes. The representative blots from three independent experiments are shown.

    Techniques Used: Biomarker Discovery, Over Expression, Expressing, Quantitative RT-PCR, Control, Transfection, Plasmid Preparation

    Heatmap of the differentially expressed genes in CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines transfected with empty vector or ERBB2, either clustered based on expression ( A ) or grouped based on transfection and phenotype ( B ).
    Figure Legend Snippet: Heatmap of the differentially expressed genes in CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines transfected with empty vector or ERBB2, either clustered based on expression ( A ) or grouped based on transfection and phenotype ( B ).

    Techniques Used: Transfection, Plasmid Preparation, Expressing

    Genome-wide gene expression changes between ERBB2 + and ERBB2 − CRC patients. ( A ) Principal component analysis (PCA) was performed to determine batch effects among the 14 patients’ samples. Comparison of PC1 and PC2 variation sequestered the samples based on ERBB2 expression. ( B ) Volcano plot of differentially expressed genes between ERBB2 - and ERBB2 + patients’ samples from input RNA-seq. Genes that are expressed significantly higher and lower based on log2 fold change in HER2+ samples are highlighted by green and blue dots, respectively. Unchanged transcripts are demarcated as grey circles ( p > 0.05). ( C ) Heatmap of the top 100 differentially expressed genes.
    Figure Legend Snippet: Genome-wide gene expression changes between ERBB2 + and ERBB2 − CRC patients. ( A ) Principal component analysis (PCA) was performed to determine batch effects among the 14 patients’ samples. Comparison of PC1 and PC2 variation sequestered the samples based on ERBB2 expression. ( B ) Volcano plot of differentially expressed genes between ERBB2 - and ERBB2 + patients’ samples from input RNA-seq. Genes that are expressed significantly higher and lower based on log2 fold change in HER2+ samples are highlighted by green and blue dots, respectively. Unchanged transcripts are demarcated as grey circles ( p > 0.05). ( C ) Heatmap of the top 100 differentially expressed genes.

    Techniques Used: Genome Wide, Gene Expression, Comparison, Expressing, RNA Sequencing



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    Image Search Results


    Validation of successful ectopic overexpression of ERBB2 in the CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines. ( A ) Relative ERBB2 expression in the normal colon cell lines CCD33 and CCD841 and the CRC cell lines HT29 and HCT116 as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the CCD33 cells. Data represent the mean ± SD of three independent experiments. ( B ) Relative ERBB2 expression in non-transfected and either empty pcDNA3 vector or pcDNA3- ERBB2 transfected HCT116, HT29, CCD33, and CCD841 cells as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the non-transfected cells. Data represent the mean ± SD of three independent experiments. *** p < 0.001; ns: not significant. ( C ) Same as B, but relative HER2 protein expression was determined in the different experimental conditions. Blots were re-probed with anti-β-Actin antibody to confirm equal loading across the lanes. The representative blots from three independent experiments are shown.

    Journal: Cancers

    Article Title: Transcriptomic Changes Associated with ERBB2 Overexpression in Colorectal Cancer Implicate a Potential Role of the Wnt Signaling Pathway in Tumorigenesis

    doi: 10.3390/cancers15010130

    Figure Lengend Snippet: Validation of successful ectopic overexpression of ERBB2 in the CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines. ( A ) Relative ERBB2 expression in the normal colon cell lines CCD33 and CCD841 and the CRC cell lines HT29 and HCT116 as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the CCD33 cells. Data represent the mean ± SD of three independent experiments. ( B ) Relative ERBB2 expression in non-transfected and either empty pcDNA3 vector or pcDNA3- ERBB2 transfected HCT116, HT29, CCD33, and CCD841 cells as determined by qRT-PCR. Data were normalized to the expression of the internal control 18S rRNA gene and fold expressions were plotted relative to expression in the non-transfected cells. Data represent the mean ± SD of three independent experiments. *** p < 0.001; ns: not significant. ( C ) Same as B, but relative HER2 protein expression was determined in the different experimental conditions. Blots were re-probed with anti-β-Actin antibody to confirm equal loading across the lanes. The representative blots from three independent experiments are shown.

    Article Snippet: The wild-type ERBB2 expression construct was a gift from Mien-Chie Hung (Addgene plasmid #16257; https://www.addgene.org/16257 , accessed on 15 December 2020) [ ].

    Techniques: Biomarker Discovery, Over Expression, Expressing, Quantitative RT-PCR, Control, Transfection, Plasmid Preparation

    Heatmap of the differentially expressed genes in CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines transfected with empty vector or ERBB2, either clustered based on expression ( A ) or grouped based on transfection and phenotype ( B ).

    Journal: Cancers

    Article Title: Transcriptomic Changes Associated with ERBB2 Overexpression in Colorectal Cancer Implicate a Potential Role of the Wnt Signaling Pathway in Tumorigenesis

    doi: 10.3390/cancers15010130

    Figure Lengend Snippet: Heatmap of the differentially expressed genes in CRC (HCT116 and HT29) and normal colon (CCD33 and CCD841) cell lines transfected with empty vector or ERBB2, either clustered based on expression ( A ) or grouped based on transfection and phenotype ( B ).

    Article Snippet: The wild-type ERBB2 expression construct was a gift from Mien-Chie Hung (Addgene plasmid #16257; https://www.addgene.org/16257 , accessed on 15 December 2020) [ ].

    Techniques: Transfection, Plasmid Preparation, Expressing

    Genome-wide gene expression changes between ERBB2 + and ERBB2 − CRC patients. ( A ) Principal component analysis (PCA) was performed to determine batch effects among the 14 patients’ samples. Comparison of PC1 and PC2 variation sequestered the samples based on ERBB2 expression. ( B ) Volcano plot of differentially expressed genes between ERBB2 - and ERBB2 + patients’ samples from input RNA-seq. Genes that are expressed significantly higher and lower based on log2 fold change in HER2+ samples are highlighted by green and blue dots, respectively. Unchanged transcripts are demarcated as grey circles ( p > 0.05). ( C ) Heatmap of the top 100 differentially expressed genes.

    Journal: Cancers

    Article Title: Transcriptomic Changes Associated with ERBB2 Overexpression in Colorectal Cancer Implicate a Potential Role of the Wnt Signaling Pathway in Tumorigenesis

    doi: 10.3390/cancers15010130

    Figure Lengend Snippet: Genome-wide gene expression changes between ERBB2 + and ERBB2 − CRC patients. ( A ) Principal component analysis (PCA) was performed to determine batch effects among the 14 patients’ samples. Comparison of PC1 and PC2 variation sequestered the samples based on ERBB2 expression. ( B ) Volcano plot of differentially expressed genes between ERBB2 - and ERBB2 + patients’ samples from input RNA-seq. Genes that are expressed significantly higher and lower based on log2 fold change in HER2+ samples are highlighted by green and blue dots, respectively. Unchanged transcripts are demarcated as grey circles ( p > 0.05). ( C ) Heatmap of the top 100 differentially expressed genes.

    Article Snippet: The wild-type ERBB2 expression construct was a gift from Mien-Chie Hung (Addgene plasmid #16257; https://www.addgene.org/16257 , accessed on 15 December 2020) [ ].

    Techniques: Genome Wide, Gene Expression, Comparison, Expressing, RNA Sequencing